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KMID : 0364820140500030201
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Korean Journal of Microbiology
2014 Volume.50 No. 3 p.201 ~ p.209
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Biological Control of Anthracnose (Colletotrichum gloeosporioides) in Red Pepper by Bacillus sp. CS-52
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Kwon Jung-Ja
Lee Jung-Bok
Kim Byum Soo
Lee Eun-Ho
Kang Kyeong-Muk
Shim Jang-Sub
Joo Woo-Hong
Jeon Chun-pyo
Kwon Gi-Seok
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Abstract
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This study was carried out in order to develop a biological control of anthracnose of red pepper caused by fungal
pathogens. In particular, this study focuses on the Colletotrichum species, which includes important fungal
pathogens causing a great deal of damage to red pepper. Antagonistic bacteria were isolated from the soil of pepper
fields, which were then tested for biocontrol activity against the Colletotrichum gloeosporioides anthracnose
pathogen of pepper. Based on the 16S rRNA sequence analysis, the isolated bacterial strain CS-52 was identical to
Bacillus sp. The culture broth of Bacillus sp. CS-52 had antifungal activity toward the hyphae and spores of C.
gloeosporioides. Moreover, the substances with antifungal activity were optimized when Bacillus sp. CS-52 was
grown aerobically in a medium composed of 0.5% glucose, 0.7% K2HPO4, 0.2% KH2PO4, 0.3% NH4NO3, 0.01%
MnSO4·7H2O, and 0.15% yeast extract at 30℃. The inhibition of spore formation resulting from cellulase,
siderophores, and indole-3-acetic acid (IAA), were produced at 24 h, 48 h, and 72 h, respectively. Bacillus sp. CS-52
also exhibited its potent fungicidal activity against anthracnose in an in vivo test, at a level of 70% when compared
to chemical fungicides. These results identified substances with antifungal activity produced by Bacillus sp. CS-52
for the biological control of major plant pathogens in red pepper. Further studies will investigate the synergistic
effect promoting better growth and antifungal activity by the formulation of substances with antifungal activity.
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KEYWORD
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Bacillus sp. CS-52, Colletotrichum gloeosporioides, antagonistic microorganism, antifungal activity, red pepper anthracnose
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